ST14 Kit ELISA

N° du produit ABIN2648780

Détail du produit ST14 Kit ELISA

Antigène: ST14 (Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum

Sensibilité: 2 ng/ml

Attributs du produit: The protein encoded by the Matriptase gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion and metastasis.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The Matriptase (TADG-15) ELISA assay kit is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact Matriptase molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a biotin conjugated anti-Matriptase monoclonal antibody. After a second wash Streptavidin conjugated to HRP is added as a reporting agent. Excess streptavidin-HRP is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if Matriptase is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of Matriptase is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: ST14 (Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))

Autre désignation: Matriptase (ST14 Produits)

Synonymes: HAI Kit ELISA, MT-SP1 Kit ELISA, MTSP1 Kit ELISA, PRSS14 Kit ELISA, SNC19 Kit ELISA, TADG15 Kit ELISA, TMPRSS14 Kit ELISA, XMT-SP1 Kit ELISA, hai Kit ELISA, mt-sp1 Kit ELISA, mtsp1 Kit ELISA, prss14 Kit ELISA, snc19 Kit ELISA, st14 Kit ELISA, st14a Kit ELISA, tadg15 Kit ELISA, tmprss1 Kit ELISA, Epithin Kit ELISA, Prss14 Kit ELISA, Tmprss14 Kit ELISA, mCAP3 Kit ELISA, matriptase Kit ELISA, suppression of tumorigenicity 14 Kit ELISA, suppression of tumorigenicity 14 L homeolog Kit ELISA, suppression of tumorigenicity 14 (colon carcinoma) Kit ELISA, ST14 Kit ELISA, st14.L Kit ELISA, St14 Kit ELISA