Serotonin Kit ELISA

N° du produit ABIN2648821

Détail du produit Serotonin Kit ELISA

Antigène: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivité: Chemical

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Urine, Cerebrospinal Fluid

Sensibilité: 5 ng/mL

Attributs du produit: Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain. Central-serotonergic neurons influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 10 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The competitive Serotonin ELISA kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Autre désignation: Serotonin (5-HT Produits)

Classe de substances: Chemical