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ADAMTS1 Kit ELISA
ADAMTS1
Reactivité: Humain, Souris
Colorimetric
Sandwich ELISA
0.4-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702804
Détail du produit ADAMTS1 Kit ELISA
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Antigène
Voir toutes ADAMTS1 Kits ELISA
ADAMTS1
(ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
Reactivité
Toutes les réactivités sur ADAMTS1 Kits ELISA
Humain, Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.4-100 ng/mL
Seuil minimal de détection
0.4 ng/mL
Application
ELISA
Fonction
Mouse ADAMTS-1 (METH1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects mouse and human ADAMTS1. Other species not determined.
Sensibilité
0.4 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes ADAMTS1 Kits ELISA
ADAMTS1
(ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
Autre désignation
ADAMTS-1 (ADAMTS1 Produits )
Synonymes
c3-c5 Kit ELISA, meth1 Kit ELISA, xadamts1 Kit ELISA, C3-C5 Kit ELISA, METH1 Kit ELISA, ADAM-TS1 Kit ELISA, ADAMTS Kit ELISA, ADAMTS-1 Kit ELISA, METH-1 Kit ELISA, adamts1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif, 1 Kit ELISA, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 L homeolog Kit ELISA, ADAMTS1 Kit ELISA, adamts1 Kit ELISA, Adamts1 Kit ELISA, adamts1.L Kit ELISA
Sujet
Gene Names: Adamts1 Protein names: A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAM-TS 1) (ADAM-TS1) (ADAMTS-1) (EC 3.4.24.-)
ID gène
11504
UniProt
P97857
Pathways
SARS-CoV-2 Protein Interactome
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