APOA2 Kit ELISA
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- Antigène Voir toutes APOA2 Kits ELISA
- APOA2 (Apolipoprotein A-II (APOA2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.025-20 ng/mL
- Seuil minimal de détection
- 0.025 ng/mL
- Application
- ELISA
- Fonction
- Human ApoA2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human ApoA2. Other species not determined.
- Sensibilité
- 0.025 ng/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples100,000 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Préparation de l'échantillon
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For example, add 2 μL of serum or plasma into a tube with 398 μL 1X Assay Diluent to prepare a 200-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 200-fold diluted sample into a tube with 998 μL 1X Assay Diluent to prepare a final 100,000-fold diluted sample.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes APOA2 Kits ELISA
- APOA2 (Apolipoprotein A-II (APOA2))
- Autre désignation
- ApoA2 (APOA2 Produits)
- Synonymes
- Apo-AII Kit ELISA, ApoA-II Kit ELISA, apoAII Kit ELISA, Alp-2 Kit ELISA, ApoAII Kit ELISA, Apoa-2 Kit ELISA, Hdl-1 Kit ELISA, APOAII Kit ELISA, apoa2 Kit ELISA, apoA-II Kit ELISA, cb1032 Kit ELISA, wu:fb57h11 Kit ELISA, zgc:193613 Kit ELISA, apolipoprotein A2 Kit ELISA, apolipoprotein A-II Kit ELISA, APOA2 Kit ELISA, Apoa2 Kit ELISA, apoa2 Kit ELISA
- Sujet
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Gene Names: APOA2
Protein names: Apolipoprotein A-II (Apo-AII) (ApoA-II) (Apolipoprotein A2) [Cleaved into: Proapolipoprotein A-II (ProapoA-II), Truncated apolipoprotein A-II (Apolipoprotein A-II(1-76))]
- ID gène
- 336
- UniProt
- P02652
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism
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