APOC3 Kit ELISA
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- Antigène Voir toutes APOC3 Kits ELISA
- APOC3 (Apolipoprotein C-III (APOC3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 2.5-10.000 pg/mL
- Seuil minimal de détection
- 2.5 pg/mL
- Application
- ELISA
- Fonction
- Human ApoC3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
- Sensibilité
- 2.5 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples500,000 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Préparation de l'échantillon
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For example, add 2 μL of serum/plasma into a tube with 998.0 μL Assay Diluent to prepare a 500-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 500-fold diluted sample into a tube with 1998 μL Assay Diluent to prepare a final 500,000 fold diluted sample.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes APOC3 Kits ELISA
- APOC3 (Apolipoprotein C-III (APOC3))
- Autre désignation
- ApoC3 (APOC3 Produits)
- Synonymes
- APOC3 Kit ELISA, APOCIII Kit ELISA, HALP2 Kit ELISA, Apoc Kit ELISA, ApoC-III Kit ELISA, apo-CIII Kit ELISA, Apo-CIII Kit ELISA, apolipoprotein C3 Kit ELISA, apolipoprotein C-III Kit ELISA, APOC3 Kit ELISA, Apoc3 Kit ELISA
- Sujet
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Gene Names: APOC3
Protein names: Apolipoprotein C-III (Apo-CIII) (ApoC-III) (Apolipoprotein C3)
- ID gène
- 345
- UniProt
- P02656
- Pathways
- Carbohydrate Homeostasis, Lipid Metabolism
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