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Factor VII Kit ELISA
F7
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.4-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702923
Détail du produit Factor VII Kit ELISA
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Antigène
Voir toutes Factor VII (F7) Kits ELISA
Factor VII (F7)
(Coagulation Factor VII (F7))
Reactivité
Toutes les réactivités sur Factor VII Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.4-100 ng/mL
Seuil minimal de détection
0.4 ng/mL
Application
ELISA
Fonction
Human Coagulation Factor VII ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Human Coagulation Factor VII (F7)
Sensibilité
0.4 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Factor VII (F7) Kits ELISA
Factor VII (F7)
(Coagulation Factor VII (F7))
Autre désignation
Coagulation Factor VII (F7 Produits )
Synonymes
F7 Kit ELISA, wu:fb59f04 Kit ELISA, zgc:109870 Kit ELISA, SPCA Kit ELISA, AI114908 Kit ELISA, ATIII Kit ELISA, At-3 Kit ELISA, At3 Kit ELISA, LOC100223776 Kit ELISA, AI132620 Kit ELISA, Cf7 Kit ELISA, FVII Kit ELISA, mfVII Kit ELISA, f7 Kit ELISA, coagulation factor VII Kit ELISA, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Kit ELISA, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog Kit ELISA, F7 Kit ELISA, f7 Kit ELISA, Serpinc1 Kit ELISA, CpipJ_CPIJ009142 Kit ELISA, CpipJ_CPIJ010295 Kit ELISA, CpipJ_CPIJ020127 Kit ELISA, fa7 Kit ELISA, f7.S Kit ELISA
Sujet
Gene Names: F7 Protein names: Coagulation factor VII (EC 3.4.21.21) (Proconvertin) (Serum prothrombin conversion accelerator) (SPCA) (Eptacog alfa) [Cleaved into: Factor VII light chain, Factor VII heavy chain]
ID gène
2155
UniProt
P08709
Pathways
Response to Growth Hormone Stimulus , Platelet-derived growth Factor Receptor Signaling
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