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SPINT1 Kit ELISA
SPINT1
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.8-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703070
Détail du produit SPINT1 Kit ELISA
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Antigène
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SPINT1
(serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))
Reactivité
Toutes les réactivités sur SPINT1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.8-200 ng/mL
Seuil minimal de détection
0.8 ng/mL
Application
ELISA
Fonction
Human HAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects human HAI-1, no cross-reactivity with mouse HAI-1, human APP, GASP-1, GASP-2, HAI-2A, TFPI, TFPI-2 is observed.
Sensibilité
0.8 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SPINT1 Kits ELISA
SPINT1
(serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))
Autre désignation
HAI-1 (SPINT1 Produits )
Synonymes
HAI-1 Kit ELISA, HAI Kit ELISA, HAI1 Kit ELISA, MANSC2 Kit ELISA, cb376 Kit ELISA, sb:cb376 Kit ELISA, spint1l Kit ELISA, zgc:64075 Kit ELISA, serine peptidase inhibitor, Kunitz type 1 Kit ELISA, serine protease inhibitor, Kunitz type 1 Kit ELISA, serine peptidase inhibitor, Kunitz type 1 b Kit ELISA, serine peptidase inhibitor, Kunitz type 1 a Kit ELISA, SPINT1 Kit ELISA, Spint1 Kit ELISA, spint1b Kit ELISA, spint1a Kit ELISA
Sujet
Gene Names: SPINT1 HAI1 UNQ223/PRO256 Protein names: Kunitz-type protease inhibitor 1 (Hepatocyte growth factor activator inhibitor type 1) (HAI-1)
ID gène
6692
UniProt
O43278
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