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IFNAR2 Kit ELISA
IFNAR2
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.16-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703098
Détail du produit IFNAR2 Kit ELISA
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Antigène
Voir toutes IFNAR2 Kits ELISA
IFNAR2
(Interferon alpha/beta Receptor 2 (IFNAR2))
Reactivité
Toutes les réactivités sur IFNAR2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.16-40 ng/mL
Seuil minimal de détection
0.16 ng/mL
Application
ELISA
Fonction
Human IFN-alpha / beta R2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM9, CD244, APRIL, BMP-2, BMP-9, Cathepsin L, CD200, CD97, Chemerin, C5a, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, FABP2, FAP, FGF-19, IFG-2R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PG-1, PGRPs, SerpinA4, sFRP-3, Thrombomodulin, TLR2, Transferrin, WIF-1
Sensibilité
0.16 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IFNAR2 Kits ELISA
IFNAR2
(Interferon alpha/beta Receptor 2 (IFNAR2))
Autre désignation
IFN alpha/beta R2 (IFNAR2 Produits )
Synonymes
AI747302 Kit ELISA, Ifnar-2 Kit ELISA, IFN-R Kit ELISA, IFN-alpha-REC Kit ELISA, IFNABR Kit ELISA, IFNARB Kit ELISA, IFN-R-2 Kit ELISA, IFNBR Kit ELISA, interferon alpha and beta receptor subunit 2 Kit ELISA, interferon (alpha and beta) receptor 2 Kit ELISA, IFNAR2 Kit ELISA, Ifnar2 Kit ELISA
Sujet
Gene Names: IFNAR2 IFNABR IFNARB Protein names: Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2)
ID gène
3455
UniProt
P48551
Pathways
Signalistation JAK/STAT , Hepatitis C
Vus récemment
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