IL12 Kit ELISA
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- Antigène Voir toutes IL12 Kits ELISA
- IL12 (Interleukin 12 (IL12))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 5-2000 pg/mL
- Seuil minimal de détection
- 5 pg/mL
- Application
- ELISA
- Fonction
- Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
- Sensibilité
- 5 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling IL12 Kit ELISA
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- Discover our top product IL12 Kit ELISA
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- Indications d'application
- Recommended Dilution for serum and plasma samples10 - 100 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." dans: Journal of neurochemistry, (2015) (PubMed).
: "Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling." dans: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 27, Issue 3, pp. 347-55, (2015) (PubMed).
: "Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine." dans: Cellular signalling, Vol. 27, Issue 7, pp. 1398-412, (2015) (PubMed).
: "Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophages and possible mechanisms of CCR-2/TLR-2 and MCP-1 signalling during Staphylococcus aureus infection." dans: Immunobiology, Vol. 220, Issue 3, pp. 350-62, (2015) (PubMed).
: "Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes." dans: Frontiers in pharmacology, Vol. 6, pp. 56, (2015) (PubMed).
: "Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes." dans: Frontiers in pharmacology, Vol. 6, pp. 74, (2015) (PubMed).
: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." dans: Blood, Vol. 121, Issue 6, pp. 930-9, (2013) (PubMed).
: "Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis." dans: Experimental dermatology, Vol. 21, Issue 9, pp. 714-5, (2012) (PubMed).
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Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." dans: Journal of neurochemistry, (2015) (PubMed).
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- Antigène Voir toutes IL12 Kits ELISA
- IL12 (Interleukin 12 (IL12))
- Autre désignation
- IL-12 p40/70 (IL12 Produits)
- Synonymes
- CLMF Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12 Kit ELISA, LOC100217488 Kit ELISA, IL-12p35 Kit ELISA, Il-12a Kit ELISA, Ll12a Kit ELISA, p35 Kit ELISA, IL12p40 Kit ELISA, IL12B Kit ELISA, IL-12 p35 Kit ELISA, interleukin 12B Kit ELISA, interleukin 12A Kit ELISA, interleukin 12b Kit ELISA, interleukin 12a Kit ELISA, Interleukin 12 level Kit ELISA, IL12B Kit ELISA, IL12A Kit ELISA, Il12b Kit ELISA, Il12a Kit ELISA, IL12 Kit ELISA, il12a Kit ELISA
- Sujet
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Gene Names: Il12b
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
- ID gène
- 16160
- UniProt
- P43432
- Pathways
- Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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