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IL15RA Kit ELISA
IL15RA
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.08-20 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703178
Détail du produit IL15RA Kit ELISA
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Antigène
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IL15RA
(Interleukin 15 Receptor, alpha (IL15RA))
Reactivité
Toutes les réactivités sur IL15RA Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.08-20 ng/mL
Seuil minimal de détection
0.08 ng/mL
Application
ELISA
Fonction
Human IL-15 R alpha ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Human Interleukin 15 Receptor alpha (IL15RA)
Sensibilité
0.08 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IL15RA Kits ELISA
IL15RA
(Interleukin 15 Receptor, alpha (IL15RA))
Autre désignation
IL-15 R alpha (IL15RA Produits )
Synonymes
IL15RA Kit ELISA, CD215 Kit ELISA, AA690181 Kit ELISA, IL-15RA Kit ELISA, interleukin 15 receptor subunit alpha Kit ELISA, interleukin 15 receptor, alpha chain Kit ELISA, IL15RA Kit ELISA, Il15ra Kit ELISA
Sujet
Gene Names: IL15RA Protein names: Interleukin-15 receptor subunit alpha (IL-15 receptor subunit alpha) (IL-15R-alpha) (IL-15RA) (CD antigen CD215) [Cleaved into: Soluble interleukin-15 receptor subunit alpha (sIL-15 receptor subunit alpha) (sIL-15R-alpha) (sIL-15RA)]
ID gène
3601
UniProt
Q13261
Pathways
Signalistation JAK/STAT
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