IL23 Kit ELISA
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- Antigène Voir toutes IL23 Kits ELISA
- IL23 (Interleukin 23 (IL23))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 25-7000 pg/mL
- Seuil minimal de détection
- 25 pg/mL
- Application
- ELISA
- Fonction
- Human IL-23 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human IL-23. Other species not determined.
- Sensibilité
- 25 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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-
Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." dans: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).
: "
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Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." dans: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).
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- Antigène Voir toutes IL23 Kits ELISA
- IL23 (Interleukin 23 (IL23))
- Autre désignation
- IL-23 (IL23 Produits)
- Synonymes
- IL-23 Kit ELISA, p19 Kit ELISA, SGRF Kit ELISA, il23p19 Kit ELISA, interleukin 23, alpha subunit p19 Kit ELISA, interleukin 23 subunit alpha Kit ELISA, Il23a Kit ELISA, IL23A Kit ELISA
- Sujet
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Gene Names: IL23A SGRF UNQ2498/PRO5798
Protein names: Interleukin-23 subunit alpha (IL-23 subunit alpha) (IL-23-A) (Interleukin-23 subunit p19) (IL-23p19)
- ID gène
- 51561
- UniProt
- Q9NPF7
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