IL-34 Kit ELISA

N° du produit ABIN2703233

Détail du produit IL-34 Kit ELISA

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7-250 pg/mL

Seuil minimal de détection: 7 pg/mL

Application: ELISA

Fonction: Human IL-34 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA4, Dkk-4 , DPPIV, EDA-A2, Epo R, FGF-6, FGF-9, Gas1, IGFBP-5, IL-1F5, IL-1F6, IL-1F7, IL-1F8, IL-1F9, IL-1F10, IL-1R5, IL-17C, IL-18, IL-20, IL-5 R alpha, IL-10 R alpha, Layilin , Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, PSMA, SIGIRR, TGF-beta RIII, Tissue Factor, TWEAK.

Sensibilité: 7 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Autre désignation: IL-34 (IL34 Produits)

Sujet: Gene Names: IL34 C16orf77
Protein names: Interleukin-34 (IL-34)

ID gène: 146433

UniProt: Q6ZMJ4

Pathways: Signalisation RTK