IL-5 Kit ELISA
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- Antigène Voir toutes IL-5 (IL5) Kits ELISA
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1-500 pg/mL
- Seuil minimal de détection
- 1 pg/mL
- Application
- ELISA
- Fonction
- Mouse IL-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
- Sensibilité
- 1 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling IL5 Kit ELISA
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice." dans: Experimental & applied acarology, Vol. 63, Issue 3, pp. 401-12, (2014) (PubMed).
: "Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis." dans: Experimental dermatology, Vol. 21, Issue 9, pp. 714-5, (2012) (PubMed).
: "Short-term consequences of reproductive mode variation on the genetic architecture of energy metabolism and life-history traits in the pea aphid." dans: Journal of insect physiology, Vol. 57, Issue 7, pp. 986-94, (2011) (PubMed).
: "C-fibers, but not the transient potential receptor vanilloid 1 (TRPV1), play a role in experimental allergic airway inflammation." dans: European journal of pharmacology, Vol. 662, Issue 1-3, pp. 55-62, (2011) (PubMed).
: "
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Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice." dans: Experimental & applied acarology, Vol. 63, Issue 3, pp. 401-12, (2014) (PubMed).
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- Antigène Voir toutes IL-5 (IL5) Kits ELISA
- IL-5 (IL5) (Interleukin 5 (IL5))
- Autre désignation
- IL-5 (IL5 Produits)
- Synonymes
- EDF Kit ELISA, IL-5 Kit ELISA, TRF Kit ELISA, Il-5 Kit ELISA, IL5 Kit ELISA, interleukin 5 Kit ELISA, IL5 Kit ELISA, Il5 Kit ELISA
- Sujet
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Gene Names: Il5 Il-5
Protein names: Interleukin-5 (IL-5) (B-cell growth factor II) (BCGF-II) (Cytotoxic T-lymphocyte inducer) (Eosinophil differentiation factor) (T-cell replacing factor) (TRF)
- ID gène
- 16191
- UniProt
- P04401
- Pathways
- Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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