Kallikrein 6 Kit ELISA
-
- Antigène Voir toutes Kallikrein 6 (KLK6) Kits ELISA
- Kallikrein 6 (KLK6)
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.6-150 ng/mL
- Seuil minimal de détection
- 0.6 ng/mL
- Application
- ELISA
- Fonction
- Human Kallikrein 6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human KLK6. Other species not determined.
- Sensibilité
- 0.6 ng/mL
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling KLK6 Kit ELISA
- Top Product
- Discover our top product KLK6 Kit ELISA
-
-
- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
-
-
Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." dans: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).
: "
-
Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." dans: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).
-
- Antigène Voir toutes Kallikrein 6 (KLK6) Kits ELISA
- Kallikrein 6 (KLK6)
- Autre désignation
- KLK6 (KLK6 Produits)
- Synonymes
- Bssp Kit ELISA, Klk7 Kit ELISA, PRSS18 Kit ELISA, PRSS9 Kit ELISA, SP59 Kit ELISA, hK6 Kit ELISA, Prss9 Kit ELISA, AI849898 Kit ELISA, BSP Kit ELISA, Klk29 Kit ELISA, MSP Kit ELISA, Prss18 Kit ELISA, neurosin Kit ELISA, kallikrein related peptidase 6 Kit ELISA, kallikrein related-peptidase 6 Kit ELISA, kallikrein-6 Kit ELISA, KLK6 Kit ELISA, Klk6 Kit ELISA, LOC100716801 Kit ELISA
- Sujet
-
Gene Names: KLK6 PRSS18 PRSS9
Protein names: Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)
- ID gène
- 5653
- UniProt
- Q92876
- Pathways
- Système du Complément, Regulation of G-Protein Coupled Receptor Protein Signaling
-