Thymidine Phosphorylase Kit ELISA
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- Antigène Voir toutes Thymidine Phosphorylase (TYMP) Kits ELISA
- Thymidine Phosphorylase (TYMP)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.4-100 ng/mL
- Seuil minimal de détection
- 0.4 ng/mL
- Application
- ELISA
- Fonction
- Human PD-ECGF (Thymidine Phosphorylase) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human PD-ECGF. Other species not determined.
- Sensibilité
- 0.4 ng/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Thymidine Phosphorylase (TYMP) Kits ELISA
- Thymidine Phosphorylase (TYMP)
- Autre désignation
- PD-ECGF (TYMP Produits)
- Synonymes
- ECGF Kit ELISA, ECGF1 Kit ELISA, MEDPS1 Kit ELISA, MNGIE Kit ELISA, MTDPS1 Kit ELISA, PDECGF Kit ELISA, TP Kit ELISA, hPD-ECGF Kit ELISA, 2900072D10Rik Kit ELISA, Ecgf1 Kit ELISA, PD-ECGF Kit ELISA, Pdgfec Kit ELISA, typh Kit ELISA, thymidine phosphorylase Kit ELISA, thymidine phosphorylase DeoA Kit ELISA, AMP phosphorylase Kit ELISA, Thymidine phosphorylase Kit ELISA, TYMP Kit ELISA, Tymp Kit ELISA, deoA Kit ELISA, Dd586_0614 Kit ELISA, Kvar_4413 Kit ELISA, NMAG_RS19330 Kit ELISA, Snov_1187 Kit ELISA, Fbal_2856 Kit ELISA, Ftrac_2071 Kit ELISA, Intca_1216 Kit ELISA, ARCVE_RS10415 Kit ELISA, Sphch_4174 Kit ELISA, Sinme_3461 Kit ELISA, METOK_RS06355 Kit ELISA, Mesop_1831 Kit ELISA, typh Kit ELISA
- Sujet
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Gene Names: TYMP ECGF1
Protein names: Thymidine phosphorylase (TP) (EC 2.4.2.4) (Gliostatin) (Platelet-derived endothelial cell growth factor) (PD-ECGF) (TdRPase)
- ID gène
- 1890
- UniProt
- P19971
- Pathways
- Signaling Events mediated by VEGFR1 and VEGFR2
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