PRSS8 Kit ELISA
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- Antigène Voir toutes PRSS8 Kits ELISA
- PRSS8 (Protease, serine, 8 (PRSS8))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 30-8000 pg/mL
- Seuil minimal de détection
- 30 pg/mL
- Application
- ELISA
- Fonction
- Human Prostasin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4, DKK-4, DPPIV, EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR, IGFBP-5, IL-1 F5, IL-1 F6, IL-1 F7, IL-1 F8, IL-1 F9, IL-1 F10, IL-1 R5, IL-21, IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, PSMA, SIGIRR, TGF beta R3, Coagulation Factor III, TWEAK.
- Sensibilité
- 30 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes PRSS8 Kits ELISA
- PRSS8 (Protease, serine, 8 (PRSS8))
- Autre désignation
- Prostasin (PRSS8 Produits)
- Synonymes
- CAP1 Kit ELISA, PROSTASIN Kit ELISA, prostasin Kit ELISA, 2410039E18Rik Kit ELISA, AI313909 Kit ELISA, C79772 Kit ELISA, fr Kit ELISA, mCAP1 Kit ELISA, protease, serine 8 Kit ELISA, protease, serine, 8 Kit ELISA, protease, serine 8 (prostasin) Kit ELISA, PRSS8 Kit ELISA, Prss8 Kit ELISA
- Sujet
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Gene Names: PRSS8
Protein names: Prostasin (EC 3.4.21.-) (Channel-activating protease 1) (CAP1) (Serine protease 8) [Cleaved into: Prostasin light chain, Prostasin heavy chain]
- ID gène
- 5652
- UniProt
- Q16651
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