Resistin Kit ELISA
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- Antigène Voir toutes Resistin (RETN) Kits ELISA
- Resistin (RETN)
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Reactivité
- Chien
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 16-4000 pg/mL
- Seuil minimal de détection
- 16 pg/mL
- Application
- ELISA
- Fonction
- Canine Resistin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Canine Resistin (RETN)
- Sensibilité
- 16 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples10 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Resistin (RETN) Kits ELISA
- Resistin (RETN)
- Autre désignation
- Resistin (RETN Produits)
- Synonymes
- RETN Kit ELISA, ADSF Kit ELISA, FIZZ3 Kit ELISA, RETN1 Kit ELISA, RSTN Kit ELISA, XCP1 Kit ELISA, Fizz3 Kit ELISA, Rstn Kit ELISA, Xcp4 Kit ELISA, resistin Kit ELISA, RETN Kit ELISA, Retn Kit ELISA
- Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
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