Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions
For Research Use only
Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Ni, Wu, Peterts, Yamamoto, Qing, Nakanishi: "The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 36, pp. 12488-501, (2015) (PubMed).