SMAD1 Kit ELISA

N° du produit ABIN2747895

Détail du produit SMAD1 Kit ELISA

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Épitope: pSer463, pSer465, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-SMAD1 (SER463/465) and Total SMAD1 Kit. This assay semi-quantitatively measures phophorylated SMAD1 (Ser463/465) and Total SMAD1 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human SMAD1 phosphorylated at site Serine-463/465 and Total SMAD1.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Autre désignation: SMAD1 (SMAD1 Produits)

Synonymes: MLP Kit ELISA, Xmad1 Kit ELISA, Xsmad1 Kit ELISA, bsp1 Kit ELISA, jv4-1 Kit ELISA, jv41 Kit ELISA, madh1 Kit ELISA, madr1 Kit ELISA, smad-1 Kit ELISA, MADH1 Kit ELISA, fb39h09 Kit ELISA, wu:fb39h09 Kit ELISA, wu:fb97e04 Kit ELISA, 2/23 Kit ELISA, CG12399 Kit ELISA, Dmel\\CG12399 Kit ELISA, E(zen)2 Kit ELISA, En(vvl) Kit ELISA, MAD Kit ELISA, Mat Kit ELISA, P-Mad Kit ELISA, P-mad Kit ELISA, PMad Kit ELISA, Smad Kit ELISA, Smad1 Kit ELISA, apg Kit ELISA, c28 Kit ELISA, dMAD Kit ELISA, dMad Kit ELISA, l(2)K00237 Kit ELISA, l(2)k00237 Kit ELISA, mad Kit ELISA, p-Mad Kit ELISA, pMAD Kit ELISA, pMad Kit ELISA, pSmad Kit ELISA, BSP-1 Kit ELISA, BSP1 Kit ELISA, JV4-1 Kit ELISA, JV41 Kit ELISA, MADR1 Kit ELISA, AI528653 Kit ELISA, Madh1 Kit ELISA, Madr1 Kit ELISA, Mlp1 Kit ELISA, MusMLP Kit ELISA, smad1 Kit ELISA, xmad Kit ELISA, SMAD family member 1 Kit ELISA, Mothers against dpp Kit ELISA, SMAD family member 1 L homeolog Kit ELISA, smad1 Kit ELISA, SMAD1 Kit ELISA, Mad Kit ELISA, Smad1 Kit ELISA, smad1.L Kit ELISA

Sujet: Mothers against decapentaplegic homolog 1 (SMAD1) phosphorylated at Serine-463/465 and Total SMAD1

ID gène: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development