SMAD2 Kit ELISA

N° du produit ABIN2747901

Détail du produit SMAD2 Kit ELISA

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Épitope: pSer245, pSer250, pSer255, total

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) and Total SMAD2 Kit. This assay semi-quantitatively measures phophorylated SMAD2 (Ser245/250/255) and Total SMAD2 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse, rat SMAD2 phosphorylated at site Serine-245/250/255 and Total SMAD2.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Autre désignation: SMAD2 (SMAD2 Produits)

Synonymes: JV18 Kit ELISA, JV18-1 Kit ELISA, MADH2 Kit ELISA, MADR2 Kit ELISA, hMAD-2 Kit ELISA, hSMAD2 Kit ELISA, jv18 Kit ELISA, Xmad2 Kit ELISA, madh2 Kit ELISA, madr2 Kit ELISA, XSmad2 Kit ELISA, hmad-2 Kit ELISA, hsmad2 Kit ELISA, jv18-1 Kit ELISA, smad-2 Kit ELISA, fj43c06 Kit ELISA, wu:fj43c06 Kit ELISA, 7120426M23Rik Kit ELISA, Madh2 Kit ELISA, Madr2 Kit ELISA, Smad-2 Kit ELISA, mMad2 Kit ELISA, MAD homolog 2 Kit ELISA, Mothers against DPP homolog 2 Kit ELISA, SMAD 2 Kit ELISA, Smad2 Kit ELISA, SMAD family member 2 Kit ELISA, SMAD family member 2-Z Kit ELISA, SMAD family member 2 S homeolog Kit ELISA, SMAD2 Kit ELISA, SMAD2Z Kit ELISA, smad2 Kit ELISA, smad2.S Kit ELISA, Smad2 Kit ELISA

Sujet: Mothers against decapentaplegic homolog 2 (SMAD2) phosphorylated at Serine-245/250/255 and Total SMAD2

ID gène: 4087

UniProt: Q15796

Pathways: Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus