WNK1 Kit ELISA

N° du produit ABIN2747914

Détail du produit WNK1 Kit ELISA

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Épitope: pThr60, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-WNK1 (T60) & Total WNK1 ELISA Kit. This assay semi-quantitatively measures phophorylated WNK1 (Thr60) & Total WNK1 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human WNK1 phosphorylated at site Threonine-60 and total WNK1.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Autre désignation: WNK1 (WNK1 Produits)

Synonymes: HSAN2 Kit ELISA, HSN2 Kit ELISA, KDP Kit ELISA, PRKWNK1 Kit ELISA, PSK Kit ELISA, p65 Kit ELISA, WNK1 Kit ELISA, kdp Kit ELISA, psk Kit ELISA, hsn2 Kit ELISA, hsan2 Kit ELISA, pha2c Kit ELISA, prkwnk1 Kit ELISA, 6430573H23Rik Kit ELISA, EG406236 Kit ELISA, Hsn2 Kit ELISA, Prkwnk1 Kit ELISA, mKIAA0344 Kit ELISA, ATWNK1 Kit ELISA, T9J14.14 Kit ELISA, T9J14_14 Kit ELISA, ZIK4 Kit ELISA, with no lysine (K) kinase 1 Kit ELISA, WNK lysine deficient protein kinase 1 Kit ELISA, WNK lysine deficient protein kinase 1 L homeolog Kit ELISA, serine/threonine-protein kinase WNK1 Kit ELISA, with no lysine (K) kinase 1 Kit ELISA, WNK1 Kit ELISA, wnk1.L Kit ELISA, wnk1 Kit ELISA, LOC100056745 Kit ELISA, Wnk1 Kit ELISA

Sujet: WNK Lysine-deficient Protein Kinase 1 (WNK1, KDP, PHA2C, PRKWNK1) phosphorylated at Threonine-60 & total WNK1

ID gène: 65125

UniProt: Q9H4A3