ANG Kit ELISA
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- Antigène Voir toutes ANG Kits ELISA
- ANG (Angiogenin (ANG))
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Reactivité
- Macaque rhésus
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Rhesus Macaque Angiogenin (ANG) ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Rhesus Macaque Angiogenin (ANG)
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes ANG Kits ELISA
- ANG (Angiogenin (ANG))
- Autre désignation
- Angiogenin (ANG Produits)
- Synonymes
- ALS9 Kit ELISA, HEL168 Kit ELISA, RNASE4 Kit ELISA, RNASE5 Kit ELISA, ANG Kit ELISA, AI385586 Kit ELISA, Ang1 Kit ELISA, Rnase5 Kit ELISA, Rnase5a Kit ELISA, ANG1 Kit ELISA, angiogenin Kit ELISA, angiogenin, ribonuclease, RNase A family, 5 Kit ELISA, ribonuclease A family member k6 Kit ELISA, ANG Kit ELISA, Ang Kit ELISA, RNASE6 Kit ELISA
- Sujet
- Angiogenin (ANG)
- UniProt
- Q8WN63
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