APP Kit ELISA
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- Antigène Voir toutes APP Kits ELISA
- APP (Amyloid beta (A4) Precursor Protein (APP))
- Épitope
- pThr668, total
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Phospho-APP (T668) and Total APP ELISA Kit. This assay semi-quantitatively measures phophorylated APP (Thr668) and Total APP in lysate samples.
- Type d'échantillon
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Human APP phosphorylated at site Threonine-668 and total APP.
- Attributs du produit
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Matériel non inclus
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
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- Antigène Voir toutes APP Kits ELISA
- APP (Amyloid beta (A4) Precursor Protein (APP))
- Autre désignation
- APP (APP Produits)
- Synonymes
- AAA Kit ELISA, ABETA Kit ELISA, ABPP Kit ELISA, AD1 Kit ELISA, APPI Kit ELISA, CTFgamma Kit ELISA, CVAP Kit ELISA, PN-II Kit ELISA, PN2 Kit ELISA, aaa Kit ELISA, abeta Kit ELISA, abpp Kit ELISA, ad1 Kit ELISA, appi Kit ELISA, ctfgamma Kit ELISA, cvap Kit ELISA, pn2 Kit ELISA, APP Kit ELISA, APP-like Kit ELISA, APPL Kit ELISA, Abeta Kit ELISA, BcDNA:GH04413 Kit ELISA, CG7727 Kit ELISA, Dmel\\CG7727 Kit ELISA, EG:65F1.5 Kit ELISA, appl Kit ELISA, Abpp Kit ELISA, Adap Kit ELISA, Ag Kit ELISA, Cvap Kit ELISA, E030013M08Rik Kit ELISA, betaApp Kit ELISA, app Kit ELISA, wu:fj34d10 Kit ELISA, wu:fk65e12 Kit ELISA, zgc:85740 Kit ELISA, amyloid beta precursor protein Kit ELISA, amyloid beta (A4) precursor protein Kit ELISA, beta amyloid protein precursor-like Kit ELISA, amyloid beta (A4) precursor protein a Kit ELISA, amyloid beta precursor protein L homeolog Kit ELISA, APP Kit ELISA, app Kit ELISA, Appl Kit ELISA, App Kit ELISA, appa Kit ELISA, app.L Kit ELISA
- Sujet
- Amyloid Precursor Protein (APP) phosphorylated at Threonine-668 and total APP
- ID gène
- 620
- UniProt
- P05067
- Pathways
- Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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