CXCL13 Kit ELISA

N° du produit ABIN2747988

Détail du produit CXCL13 Kit ELISA

Antigène: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Reactivité: Humain, Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 12-3000 pg/mL

Seuil minimal de détection: 12 pg/mL

Application: ELISA

Fonction: Rhesus Monkey BLC (CXCL13) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects Rhesus Monkey and Human BLC. Other species not determined.

Sensibilité: 12 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Autre désignation: BLC (CXCL13 Produits)

Synonymes: 4631412M08Rik Kit ELISA, ANGIE2 Kit ELISA, Angie Kit ELISA, BCA-1 Kit ELISA, BLC Kit ELISA, BLR1L Kit ELISA, Scyb13 Kit ELISA, ANGIE Kit ELISA, BCA1 Kit ELISA, SCYB13 Kit ELISA, C-X-C motif chemokine ligand 13 Kit ELISA, chemokine (C-X-C motif) ligand 13 Kit ELISA, Cxcl13 Kit ELISA, CXCL13 Kit ELISA

Sujet: Tyrosine-protein kinase Blk

UniProt: H9ZC88