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CNTF Receptor alpha Kit ELISA

CNTFR Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN2748027
  • Antigène Voir toutes CNTF Receptor alpha (CNTFR) Kits ELISA
    CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))
    Reactivité
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    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat CNTF R alpha ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Rat CNTF R alpha. Other species not yet determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CNTFR Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CNTF Receptor alpha (CNTFR) Kits ELISA
    CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))
    Autre désignation
    CNTF R alpha (CNTFR Produits)
    Synonymes
    CNTFR-alpha Kit ELISA, CNTFRALPHA Kit ELISA, Cntfralpha Kit ELISA, cb549 Kit ELISA, fj33e02 Kit ELISA, sb:cb549 Kit ELISA, wu:fj33e02 Kit ELISA, si:bz76a6.1 Kit ELISA, si:dz248f2.1 Kit ELISA, si:rp71-76a6.1 Kit ELISA, ciliary neurotrophic factor receptor Kit ELISA, ciliary neurotrophic factor receptor L homeolog Kit ELISA, CNTFR Kit ELISA, cntfr.L Kit ELISA, Cntfr Kit ELISA, cntfr Kit ELISA
    Sujet
    Ciliary neurotrophic factor receptor subunit alpha (CNTF receptor subunit alpha) (CNTFR-alpha)
    ID gène
    313173
    UniProt
    Q08406
    Pathways
    Signalistation JAK/STAT, Feeding Behaviour
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