ERBB3 Kit ELISA

N° du produit ABIN2748081

Détail du produit ERBB3 Kit ELISA

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Épitope: pTyr1262

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-ErbB3 (Y1262) ELISA Kit. This assay semi-quantitatively measures phophorylated ErbB3 (Tyr1262) in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human ErbB3 phosphorylated at site Tyrosine-1262.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Autre désignation: ErbB3 (ERBB3 Produits)

Synonymes: ErbB-3 Kit ELISA, HER3 Kit ELISA, LCCS2 Kit ELISA, MDA-BF-1 Kit ELISA, c-erbB-3 Kit ELISA, c-erbB3 Kit ELISA, erbB3-S Kit ELISA, p180-ErbB3 Kit ELISA, p45-sErbB3 Kit ELISA, p85-sErbB3 Kit ELISA, ERBB3 Kit ELISA, C76256 Kit ELISA, Erbb-3 Kit ELISA, Erbb3r Kit ELISA, Her3 Kit ELISA, nuc-ErbB3 Kit ELISA, erb-b2 receptor tyrosine kinase 3 Kit ELISA, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) Kit ELISA, ERBB3 Kit ELISA, Erbb3 Kit ELISA

Sujet: ErbB3 phosphorylated at Tyrosine-1262

ID gène: 2065

UniProt: P21860

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway