ERK2 Kit ELISA

N° du produit ABIN2748087

Détail du produit ERK2 Kit ELISA

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Épitope: pThr185, pTyr187, total

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-Erk2 (T185/Y187) and Total ERK2 ELISA Kit. This assay semi-quantitatively measures phophorylated Erk2 (Thr185/Tyr187) and Total ERK2 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-ERK2 (pThr185/pTyr187) and total ERK2.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Autre désignation: Erk2 (MAPK1 Produits)

Synonymes: ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, 9030612K14Rik Kit ELISA, AA407128 Kit ELISA, AU018647 Kit ELISA, C78273 Kit ELISA, Erk2 Kit ELISA, Prkm1 Kit ELISA, p42mapk Kit ELISA, zERK2 Kit ELISA, MAPK1 Kit ELISA, Xp42 Kit ELISA, erk Kit ELISA, erk2 Kit ELISA, ert1 Kit ELISA, mapk Kit ELISA, mapk1b Kit ELISA, mapk2 Kit ELISA, mpk1 Kit ELISA, prkm1 Kit ELISA, prkm2 Kit ELISA, mapk1 Kit ELISA, mapk1a Kit ELISA, xp42 Kit ELISA, ATMPK1 Kit ELISA, F14N23.9 Kit ELISA, F14N23_9 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, MNK1 Kit ELISA, ATMPK2 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Kit ELISA, T30E16.13 Kit ELISA, T30E16_13 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, mitogen activated protein kinase 1 Kit ELISA, mitogen-activated protein kinase 1 L homeolog Kit ELISA, mitogen-activated protein kinase 1 S homeolog Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK1 Kit ELISA, Mapk1 Kit ELISA, mapk1 Kit ELISA, mapk1.L Kit ELISA, mapk1.S Kit ELISA, PfMAP1 Kit ELISA, MPK1 Kit ELISA, PVX_084965 Kit ELISA, LOC9328617 Kit ELISA, Tsp_01601 Kit ELISA, MPK2 Kit ELISA

Sujet: ERK2, Mitogen-activated protein kinase 1, MAPK1, p42MAPK

ID gène: 5594

UniProt: P28482

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins