Follistatin Kit ELISA

N° du produit ABIN2748110

Détail du produit Follistatin Kit ELISA

Antigène: Follistatin (FST)

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rhesus Macaque Follistatin (FST) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque Follistatin (FST)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Follistatin (FST)

Autre désignation: Follistatin (FST Produits)

Synonymes: CG12955 Kit ELISA, CG12956 Kit ELISA, CG33466 Kit ELISA, Dmel\\CG33466 Kit ELISA, Fol1 Kit ELISA, dFS Kit ELISA, dFol1 Kit ELISA, dfs Kit ELISA, fs Kit ELISA, FS Kit ELISA, foll Kit ELISA, xfs Kit ELISA, FST Kit ELISA, fst Kit ELISA, AL033346 Kit ELISA, FOL1 Kit ELISA, Fst-288 Kit ELISA, RATFOL1 Kit ELISA, cb446 Kit ELISA, fd07h10 Kit ELISA, fst1 Kit ELISA, wu:fd07h10 Kit ELISA, Follistatin Kit ELISA, follistatin Kit ELISA, follistatin L homeolog Kit ELISA, follistatin a Kit ELISA, Fs Kit ELISA, fst Kit ELISA, FST Kit ELISA, LOC100141662 Kit ELISA, fst.L Kit ELISA, Fst Kit ELISA, fsta Kit ELISA

Sujet: Follistatin (FST)

ID gène: 707420

UniProt: F7AVH0

Pathways: Negative Regulation of Hormone Secretion