H2AFX Kit ELISA

N° du produit ABIN2748159

Détail du produit H2AFX Kit ELISA

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Épitope: pSer139, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-H2AX (S139) and Total H2AX ELISA Kit. This assay semi-quantitatively measures phophorylated H2AX (Ser139) and Total H2AX in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human H2AX phosphorylated at Serine-139 and total H2AX.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2AX (H2AFX Produits)

Synonymes: H2A.X Kit ELISA, H2A/X Kit ELISA, H2AX Kit ELISA, AW228881 Kit ELISA, H2ax Kit ELISA, Hist5-2ax Kit ELISA, gammaH2ax Kit ELISA, zgc:56329 Kit ELISA, h2a.x Kit ELISA, h2a/x Kit ELISA, h2ax Kit ELISA, RGD1566119 Kit ELISA, h2a Kit ELISA, h2afx Kit ELISA, H2A histone family member X Kit ELISA, H2A histone family, member X Kit ELISA, H2A histone family member X L homeolog Kit ELISA, histone cluster 1, H2ah Kit ELISA, histone cluster 2, H2ab S homeolog Kit ELISA, histone H2AX Kit ELISA, H2AFX Kit ELISA, H2afx Kit ELISA, h2afx Kit ELISA, h2afx.L Kit ELISA, HIST1H2AH Kit ELISA, hist2h2ab.S Kit ELISA, LOC100522201 Kit ELISA, LOC100720536 Kit ELISA

Sujet: Histone H2AX phosphorylated at Serine-S139 and total H2AX

ID gène: 3014

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus