JNK Kit ELISA

N° du produit ABIN2748302

Détail du produit JNK Kit ELISA

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Épitope: pThr183, pTyr185

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185).

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Références pour JNK Kit ELISA (ABIN2748302)

Bizargity, Liu, Wang, Hancock, Visner: "Inhibitory effects of pirfenidone on dendritic cells and lung allograft rejection." dans: Transplantation, Vol. 94, Issue 2, pp. 114-22, (2012) (PubMed).

Stephens, Stephens, Hobbs, Hutmacher, Bacic-Welsh, Woodruff, Morrison: "Myocyte enhancer factor 2c, an osteoblast transcription factor identified by dimethyl sulfoxide (DMSO)-enhanced mineralization." dans: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30071-86, (2011) (PubMed).

Détail du antigène

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Autre désignation: JNK (MAPK8 Produits)

Synonymes: JNK Kit ELISA, JNK-46 Kit ELISA, JNK1 Kit ELISA, JNK1A2 Kit ELISA, JNK21B1/2 Kit ELISA, PRKM8 Kit ELISA, SAPK1 Kit ELISA, SAPK1c Kit ELISA, AI849689 Kit ELISA, Prkm8 Kit ELISA, BSK Kit ELISA, BSK/DJNK Kit ELISA, Bsk Kit ELISA, CG5680 Kit ELISA, D-JNK Kit ELISA, D-junk Kit ELISA, DBSK/JNK Kit ELISA, DJNK Kit ELISA, DJNK/bsk Kit ELISA, Dmel\\CG5680 Kit ELISA, JNK/SAPK Kit ELISA, Jnk Kit ELISA, Junk Kit ELISA, SAPKa Kit ELISA, c-Jun Kit ELISA, dJNK Kit ELISA, jnk Kit ELISA, T10F20.15 Kit ELISA, jnk1 Kit ELISA, sapk1 Kit ELISA, mapk8 Kit ELISA, zgc:112379 Kit ELISA, JNKb Kit ELISA, mapk8b Kit ELISA, mitogen-activated protein kinase 8 Kit ELISA, Stress-activated protein kinase jnk-1 Kit ELISA, basket Kit ELISA, Protein kinase superfamily protein Kit ELISA, mitogen-activated protein kinase 8 L homeolog Kit ELISA, mitogen-activated protein kinase 8b Kit ELISA, mitogen-activated protein kinase 8B-like Kit ELISA, MAPK8 Kit ELISA, jnk-1 Kit ELISA, Mapk8 Kit ELISA, bsk Kit ELISA, ATMPK8 Kit ELISA, mapk8.L Kit ELISA, mapk8b Kit ELISA, LOC109094263 Kit ELISA

Sujet: C-Jun N-terminal kinase (JNK) phosphorylated at T183 and/or Y185

ID gène: 5599

UniProt: P45983

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins