LCK Kit ELISA

N° du produit ABIN2748305

Détail du produit LCK Kit ELISA

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Épitope: pTyr394, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-LCK (Y394) and Total LCK ELISA Kit. This assay semi-quantitatively measures phophorylated LCK (Tyr394) and Total LCK in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human LCK phosphorylated at Tyrosine-394 and total LCK.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.Add 100 μL of sample or positive control to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared primary antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared 1X HRP-Streptavidin to each well.Incubate 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: LCK (LCK Produits)

Synonymes: zgc:136695 Kit ELISA, LCK Kit ELISA, Hck-3 Kit ELISA, Lsk Kit ELISA, Lskt Kit ELISA, p56 Kit ELISA, p56Lck Kit ELISA, LSK Kit ELISA, YT16 Kit ELISA, p56lck Kit ELISA, pp58lck Kit ELISA, P56LCK Kit ELISA, tkl Kit ELISA, Lck1 Kit ELISA, Lcktkr Kit ELISA, LCK proto-oncogene, Src family tyrosine kinase Kit ELISA, lymphocyte protein tyrosine kinase Kit ELISA, lck Kit ELISA, LCK Kit ELISA, Lck Kit ELISA

Sujet: Lymphocyte-specific Protein Tyrosine Kinase (LCK)

ID gène: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling