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MSK2 Kit ELISA

RPS6KA4 Reactivité: Humain pSer360 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748386
  • Antigène Voir toutes MSK2 (RPS6KA4) Kits ELISA
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Épitope
    pSer360, total
    Reactivité
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-MSK2 (S360) & Total MSK2 ELISA Kit. This assay semi-quantitatively measures phophorylated MSK2 (Ser360) & Total MSK2 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human MSK2 phosphorylated at site Serine-360.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RPS6KA4 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes MSK2 (RPS6KA4) Kits ELISA
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Autre désignation
    MSK2 (RPS6KA4 Produits)
    Synonymes
    MSK2 Kit ELISA, RSK-B Kit ELISA, 1110069D02Rik Kit ELISA, 90kDa Kit ELISA, AI848992 Kit ELISA, Msk2 Kit ELISA, mMSK2 Kit ELISA, ribosomal protein S6 kinase A4 Kit ELISA, ribosomal protein S6 kinase, polypeptide 4 Kit ELISA, RPS6KA4 Kit ELISA, Rps6ka4 Kit ELISA
    Sujet
    Mitogen- and stress-activated protein kinase 2 (MSK2) phosphorylated at Serine-360 and Total MSK2
    ID gène
    8986
    UniProt
    O75676
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