Reticulon 4 Kit ELISA

N° du produit ABIN2748436

Détail du produit Reticulon 4 Kit ELISA

Antigène: Reticulon 4 (RTN4)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Reticulon 4 (RTN4)

Autre désignation: Nogo-A (RTN4 Produits)

Synonymes: ASY Kit ELISA, NI220/250 Kit ELISA, NOGO Kit ELISA, NOGO-A Kit ELISA, NOGOC Kit ELISA, NSP Kit ELISA, NSP-CL Kit ELISA, Nbla00271 Kit ELISA, Nbla10545 Kit ELISA, Nogo-B Kit ELISA, Nogo-C Kit ELISA, RTN-X Kit ELISA, RTN4-A Kit ELISA, RTN4-B1 Kit ELISA, RTN4-B2 Kit ELISA, RTN4-C Kit ELISA, RTN4-Cw Kit ELISA, DKFZp459C0314 Kit ELISA, 1110020G17Rik Kit ELISA, AA407876 Kit ELISA, AA409940 Kit ELISA, AA960376 Kit ELISA, C130026I10Rik Kit ELISA, NgA Kit ELISA, Nogo-A Kit ELISA, mKIAA0886 Kit ELISA, mKIAA4153 Kit ELISA, NI-250 Kit ELISA, Nogo Kit ELISA, Vp20 Kit ELISA, r Kit ELISA, rat N Kit ELISA, rat NogoA Kit ELISA, reticulon-4 Kit ELISA, nogo Kit ELISA, rtn4 Kit ELISA, wu:fb17a02 Kit ELISA, wu:fb22f12 Kit ELISA, wu:fd61a07 Kit ELISA, zgc:92163 Kit ELISA, reticulon 4 Kit ELISA, reticulon 4a Kit ELISA, RTN4 Kit ELISA, rtn4 Kit ELISA, Rtn4 Kit ELISA, rtn4a Kit ELISA

Sujet: Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)

ID gène: 83765

UniProt: Q9JK11

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome