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Notch1 Kit ELISA

NOTCH1 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN2748442
  • Antigène Voir toutes Notch1 (NOTCH1) Kits ELISA
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Reactivité
    • 4
    • 2
    • 2
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat Notch-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Rat Notch-1
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Notch1 (NOTCH1) Kits ELISA
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Autre désignation
    Notch-1 (NOTCH1 Produits)
    Synonymes
    notch1 Kit ELISA, NOTCH1 Kit ELISA, hn1 Kit ELISA, tan1 Kit ELISA, notch Kit ELISA, xotch Kit ELISA, xnotch Kit ELISA, notch-1 Kit ELISA, xnotch1 Kit ELISA, TAN1 Kit ELISA, hN1 Kit ELISA, 9930111A19Rik Kit ELISA, Mis6 Kit ELISA, N1 Kit ELISA, Tan1 Kit ELISA, lin-12 Kit ELISA, NOTCH Kit ELISA, Xotch Kit ELISA, notch1-a Kit ELISA, notch 1 Kit ELISA, fibropellin-1 Kit ELISA, Notch homolog 1, translocation-associated (Drosophila) Kit ELISA, Notch homolog 1, translocation-associated Kit ELISA, notch 1 S homeolog Kit ELISA, notch1 Kit ELISA, LOC100631526 Kit ELISA, NOTCH1 Kit ELISA, Notch1 Kit ELISA, notch1.S Kit ELISA
    Sujet
    Notch-1
    ID gène
    25496
    UniProt
    Q07008
    Pathways
    Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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