P-Cadherin Kit ELISA

N° du produit ABIN2748467

Détail du produit P-Cadherin Kit ELISA

Antigène: P-Cadherin (CDH3)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.041-10 ng/mL

Seuil minimal de détection: 0.041 ng/mL

Application: ELISA

Fonction: Rat P-Cadherin ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB (TNFRSF9/CD137), Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK, Decorin, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1 (GDNFR), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, Prolactin, RANTES, SCF, TIM-1 (KIM-1), TIMP-2, TREM-1, TWEAK R.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples4 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: P-Cadherin (CDH3)

Autre désignation: P-Cadherin (CDH3 Produits)

Synonymes: CDH3 Kit ELISA, Cadherin-3 Kit ELISA, AI385538 Kit ELISA, Cadp Kit ELISA, Cdhp Kit ELISA, P-cadherin Kit ELISA, Pcad Kit ELISA, CDHP Kit ELISA, HJMD Kit ELISA, PCAD Kit ELISA, E-Cad Kit ELISA, E-cadherin Kit ELISA, EP-cadherin Kit ELISA, XTCAD-1 Kit ELISA, c-cadherin Kit ELISA, cadherin-1 Kit ELISA, cdh1 Kit ELISA, cdh1-b Kit ELISA, cdh3 Kit ELISA, cdhc-A Kit ELISA, cdhp Kit ELISA, hjmd Kit ELISA, l-cam Kit ELISA, lcam Kit ELISA, pcad Kit ELISA, uvo Kit ELISA, uvomorulin Kit ELISA, xb-cad Kit ELISA, xcdh1 Kit ELISA, CDH15 Kit ELISA, K-CAM Kit ELISA, cadherin 3 Kit ELISA, cadherin 3 L homeolog Kit ELISA, Cadherin-3 Kit ELISA, CDH3 Kit ELISA, Cdh3 Kit ELISA, cdh3.L Kit ELISA, cdh-3 Kit ELISA

Sujet: P-Cadherin (CDH3 / Cadherin-3)

UniProt: Q9JIV6