CDKN1B Kit ELISA

N° du produit ABIN2748476

Détail du produit CDKN1B Kit ELISA

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat p27/Kip1 (CDKN1B) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rat p27/Kip1 (CDKN1B)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: p27/Kip1 (CDKN1B Produits)

Synonymes: CDKN4 Kit ELISA, KIP1 Kit ELISA, MEN1B Kit ELISA, MEN4 Kit ELISA, P27KIP1 Kit ELISA, cdkn1b Kit ELISA, sb:cb611 Kit ELISA, wu:fb63g10 Kit ELISA, wu:fb64g10 Kit ELISA, wu:fe18e03 Kit ELISA, CDKN1B Kit ELISA, Kip1 Kit ELISA, p27 Kit ELISA, FAM14D Kit ELISA, ISG12 Kit ELISA, ISG12A Kit ELISA, P27 Kit ELISA, Cdki1b Kit ELISA, AA408329 Kit ELISA, AI843786 Kit ELISA, p27Kip1 Kit ELISA, cdkn1bl Kit ELISA, cyclin dependent kinase inhibitor 1B Kit ELISA, cyclin-dependent kinase inhibitor 1Bb Kit ELISA, interferon alpha inducible protein 27 Kit ELISA, cyclin-dependent kinase inhibitor 1B Kit ELISA, cyclin-dependent kinase inhibitor 1Ba Kit ELISA, CDKN1B Kit ELISA, cdkn1bb Kit ELISA, IFI27 Kit ELISA, Cdkn1b Kit ELISA, cdkn1ba Kit ELISA

Sujet: P27/Kip1 (CDKN1B)

ID gène: 83571

UniProt: O08769

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy