RPS6KB1 Kit ELISA

N° du produit ABIN2748498

Détail du produit RPS6KB1 Kit ELISA

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Épitope: pSer424, pThr421, total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-P70S6K (T421/S424) and Total P70S6K ELISA Kit. This assay semi-quantitatively measures phophorylated P70S6K (Thr421/S424) and Total P70S6K in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse, and rat p70S6 Kinase phosphorylated at sites Threonine-421 and/or Serine-424 as well as total (pan) p70S6 Kinase.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: P70S6K (RPS6KB1 Produits)

Synonymes: PS6K Kit ELISA, S6K Kit ELISA, S6K-beta-1 Kit ELISA, S6K1 Kit ELISA, STK14A Kit ELISA, p70 S6KA Kit ELISA, p70(S6K)-alpha Kit ELISA, p70-S6K Kit ELISA, p70-alpha Kit ELISA, 10539 Kit ELISA, 7084 Kit ELISA, CG10539 Kit ELISA, DS6K Kit ELISA, Dmel\\CG10539 Kit ELISA, Dp70S6k Kit ELISA, Dp70[s6k] Kit ELISA, Dp70s6k Kit ELISA, S6k Kit ELISA, dS6K Kit ELISA, dS6k Kit ELISA, dp70[S6k] Kit ELISA, dp70s6k Kit ELISA, dps6k Kit ELISA, ds6k Kit ELISA, fs(3)07084 Kit ELISA, l(3)07084 Kit ELISA, p70 S6K Kit ELISA, p70/S6K Kit ELISA, p70S6K Kit ELISA, p70[S6 kinase] Kit ELISA, p70[S6K] Kit ELISA, p70[S6k] Kit ELISA, p70[S6kinase] Kit ELISA, p70s6K Kit ELISA, s6k Kit ELISA, s6k11 Kit ELISA, 2610318I15Rik Kit ELISA, 4732464A07Rik Kit ELISA, 70kDa Kit ELISA, AA959758 Kit ELISA, AI256796 Kit ELISA, AI314060 Kit ELISA, p70/85s6k Kit ELISA, p70s6k Kit ELISA, p70s6k-A Kit ELISA, p70-s6k Kit ELISA, ps6k Kit ELISA, rps6kb1 Kit ELISA, rps6kb1-A Kit ELISA, s6K1 Kit ELISA, stk14a Kit ELISA, fc51h01 Kit ELISA, wu:fc51h01 Kit ELISA, zgc:55713 Kit ELISA, ribosomal protein S6 kinase B1 Kit ELISA, Ribosomal protein S6 kinase Kit ELISA, ribosomal protein S6 kinase, polypeptide 1 Kit ELISA, ribosomal protein S6 kinase B1 L homeolog Kit ELISA, ribosomal protein S6 kinase B1 S homeolog Kit ELISA, ribosomal protein S6 kinase b, polypeptide 1b Kit ELISA, RPS6KB1 Kit ELISA, S6k Kit ELISA, Rps6kb1 Kit ELISA, rps6kb1.L Kit ELISA, rps6kb1.S Kit ELISA, rps6kb1b Kit ELISA

Sujet: P70S6 kinase (P70S6K) phosphorylated at T421 and/or S424 and total (pan) p70S6 kinase

ID gène: 6198

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg