CCL5 Kit ELISA
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- Antigène Voir toutes CCL5 Kits ELISA
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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Reactivité
- Porc
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 46.9-3000 pg/mL
- Seuil minimal de détection
- 46.9 pg/mL
- Application
- ELISA
- Fonction
- Porcine RANTES (CCL5) ELISA Kit for serum, plasma, and cell culture supernatant samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine Angiopoietin-1, IL-17F, MIF, Osteoprotegerin (TNFRSF11B), PDGF-BB, TGF alpha, TIMP-1, TIMP-2, VEGF-A
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes CCL5 Kits ELISA
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- Autre désignation
- RANTES (CCL5 Produits)
- Synonymes
- Rantes Kit ELISA, Scya5 Kit ELISA, D17S136E Kit ELISA, RANTES Kit ELISA, SCYA5 Kit ELISA, SIS-delta Kit ELISA, SISd Kit ELISA, TCP228 Kit ELISA, eoCP Kit ELISA, MuRantes Kit ELISA, C-C motif chemokine 5-like Kit ELISA, C-C motif chemokine ligand 5 Kit ELISA, chemokine (C-C motif) ligand 5 Kit ELISA, LOC100230935 Kit ELISA, CCL5 Kit ELISA, Ccl5 Kit ELISA
- Sujet
- RANTES (CCL5)
- UniProt
- Q29288
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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