RPS6 Kit ELISA

N° du produit ABIN2748617

Détail du produit RPS6 Kit ELISA

Antigène: RPS6 (Ribosomal Protein S6 (RPS6))

Épitope: pSer235, pSer236

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Type d'échantillon: Cell Culture Cells

Analytical Method: Semi-Quantitative

Specificité: The antibodies provided in this kit recognizes human/mouse/rat RPS6 phosphorylated at Serine-235 / Serine-236 and total RPS6 for comparison.

Attributs du produit:

• Site and signal pathway-specific
• In vitro detection of adherent cell culture
• No sample lysis needed
• Compatible with a standard ELISA plate reader
• Faster results than with ELISA
• Adaptable for high-throughput screening and drug discovery

Ingrédients:

• uncoated 96-well Microplate
• Wash Buffer A
• Wash Buffer B
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Uncoated

Protocole:

1. Seed 10,000-30,000 cells into each well and incubate overnight.
2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6 (Ribosomal Protein S6 (RPS6))

Autre désignation: RPS6 (RPS6 Produits)

Synonymes: S6 Kit ELISA, rps6 Kit ELISA, rps6a Kit ELISA, S6R Kit ELISA, (Rp)S6 Kit ELISA, CG10944 Kit ELISA, DS6 Kit ELISA, Dmel\\CG10944 Kit ELISA, M(1)7BC Kit ELISA, M(1)7C Kit ELISA, RPS6 Kit ELISA, Rp S6 Kit ELISA, Rps6 Kit ELISA, air8 Kit ELISA, air[8] Kit ELISA, anon-WO02059370.61 Kit ELISA, hen Kit ELISA, l(1)air8 Kit ELISA, l(1)air[8] Kit ELISA, l(1)hen Kit ELISA, pp30 Kit ELISA, rpS6 Kit ELISA, wu:fa92e06 Kit ELISA, wu:fb64g06 Kit ELISA, zgc:92237 Kit ELISA, ribosomal protein S6 Kit ELISA, ribosomal protein S6 S homeolog Kit ELISA, S6 ribosomal protein Kit ELISA, Ribosomal protein S6 Kit ELISA, 30S ribosomal protein S6 Kit ELISA, 40S ribosomal protein S6 Kit ELISA, RPS6 Kit ELISA, rps6.S Kit ELISA, LOC100135859 Kit ELISA, Rps6 Kit ELISA, RpS6 Kit ELISA, rps6 Kit ELISA, rps-6 Kit ELISA, LOC100533219 Kit ELISA

Sujet: Disease resistance protein RPS6, Resistance to Pseudomonas syringae 6 (RPS6)

ID gène: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly