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RPS6KA1 Kit ELISA

RPS6KA1 Reactivité: Humain pSer380 Colorimetric Cell ELISA Cell Culture Cells
N° du produit ABIN2748625
  • Antigène Voir toutes RPS6KA1 Kits ELISA
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Épitope
    pSer380
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based Human RSK1 (S380) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    Type d'échantillon
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibodies provided in this kit recognizes human RSK1 phosphorylated at Serine-380 and total RSK1 for comparison.
    Attributs du produit
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    Ingrédients
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Uncoated
    Protocole
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes RPS6KA1 Kits ELISA
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Autre désignation
    RSK1 (RPS6KA1 Produits)
    Synonymes
    HU-1 Kit ELISA, MAPKAPK1A Kit ELISA, RSK Kit ELISA, RSK1 Kit ELISA, Rsk1 Kit ELISA, p90rsk Kit ELISA, rsk Kit ELISA, RPS6KA Kit ELISA, hu-1 Kit ELISA, mapkapk1a Kit ELISA, rsk1 Kit ELISA, wu:fc56h07 Kit ELISA, zgc:152654 Kit ELISA, ribosomal protein S6 kinase A1 Kit ELISA, ribosomal protein S6 kinase polypeptide 1 Kit ELISA, ribosomal protein S6 kinase A1-like Kit ELISA, ribosomal protein S6 kinase A1 L homeolog Kit ELISA, ribosomal protein S6 kinase a, polypeptide 1 Kit ELISA, RPS6KA1 Kit ELISA, Rps6ka1 Kit ELISA, RPS6KA1L Kit ELISA, rps6ka1.L Kit ELISA, rps6ka1 Kit ELISA
    Sujet
    Ribosomal protein S6 kinase alpha-1, Ribosomal S6 kinase 1 (RSK1), 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a
    ID gène
    6195
    UniProt
    Q15418
    Pathways
    Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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