IL-20 Kit ELISA

N° du produit ABIN2859222

Détail du produit IL-20 Kit ELISA

Antigène: IL-20 (IL20) (Interleukin 20 (IL20))

Épitope: AA 25-176

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-20

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: L25-E176

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: L25-E176

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli, L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-20 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-20 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 285, Standard deviation: 11.97, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 709, Standard deviation: 36.16, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1126, Standard deviation: 61.93, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 343, Standard deviation: 19.55, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 762, Standard deviation: 48.77, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 1280, Standard deviation: 94.72, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-20 Kit ELISA (ABIN2859222)

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." dans: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Zhou, Jin, Jia, Zhang, Li, Liu, Liu: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." dans: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." dans: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, (2012) (PubMed).

Détail du antigène

Antigène: IL-20 (IL20) (Interleukin 20 (IL20))

Autre désignation: IL20 (IL20 Produits)

Synonymes: IL-20 Kit ELISA, IL10D Kit ELISA, ZCYTO10 Kit ELISA, Zcyto10 Kit ELISA, IL20 Kit ELISA, interleukin 20 Kit ELISA, IL20 Kit ELISA, Il20 Kit ELISA

Sujet:

Protein Function: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.

Background: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Synonyms: Interleukin-20,IL-20,Cytokine Zcyto10,Il20,Zcyto10,

Full Gene Name: Interleukin-20

Cellular Localisation: Secreted.

ID gène: 58181

UniProt: Q9JKV9

Pathways: Protein targeting to Nucleus