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Epiregulin Kit ELISA

EREG Reactivité: Humain AA 60-108 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859248
  • Antigène Voir toutes Epiregulin (EREG) Kits ELISA
    Epiregulin (EREG)
    Épitope
    AA 60-108
    Reactivité
    • 7
    • 6
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10.000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Epiregulin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    E.coli, V60-L108
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: V60-L108
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .

    Plaque
    Pre-coated
    Protocole
    human Epiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Epiregulin has been precoated onto 96-well plates. Standards (E.coli,V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Epiregulin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Epiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Epiregulin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.15, Standard deviation: 0.059, CV(%): 5.2
    • Sample 2: n=16, Mean(ng/ml): 2.52, Standard deviation: 0.113, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 5.81, Standard deviation: 0.273, CV(%): 4.7,
    • Sample 1: n=24, Mean(ng/ml): 1.63, Standard deviation: 0.096, CV(%): 5.9
    • Sample 2: n=24, Mean(ng/ml): 2.87, Standard deviation: 0.146, CV(%): 5.1
    • Sample 3: n=24, Mean(ng/ml): 5.66, Standard deviation: 0.350, CV(%): 6.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Epiregulin (EREG) Kits ELISA
    Epiregulin (EREG)
    Autre désignation
    EREG (EREG Produits)
    Synonymes
    EREG Kit ELISA, ER Kit ELISA, EPR Kit ELISA, proepiregulin Kit ELISA, epiregulin Kit ELISA, EREG Kit ELISA, ereg Kit ELISA, Ereg Kit ELISA
    Sujet

    Protein Function: Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357). .

    Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

    Synonyms: Proepiregulin,Epiregulin,EPR,EREG,

    Full Gene Name: Proepiregulin

    Cellular Localisation: Epiregulin: Secreted, extracellular space.
    ID gène
    2069
    UniProt
    O14944
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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