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IL-13 Kit ELISA

IL13 Reactivité: Humain AA 21-132 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859272
  • Antigène Voir toutes IL-13 (IL13) Kits ELISA
    IL-13 (IL13) (Interleukin 13 (IL13))
    Épitope
    AA 21-132
    Reactivité
    • 15
    • 12
    • 8
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-13
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: G21-N132
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: G21-N132
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the IL-4/IL-13 family.

    Plaque
    Pre-coated
    Protocole
    human IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, G21-N132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-13 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-13 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-13 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 98, Standard deviation: 4.02, CV(%): 4.1
    • Sample 2: n=16, Mean(pg/ml): 340, Standard deviation: 10.88, CV(%): 3.2
    • Sample 3: n=16, Mean(pg/ml): 497, Standard deviation: 19.38, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 115, Standard deviation: 8.28, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 19.82, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 512, Standard deviation: 29.7, CV(%): 5.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhang, Huang, Jin, Han: "Therapeutic Effects of a Long-Acting Cholinergic Receptor Blocker, Tiotropium Bromide, on Asthma." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 944-950, (2018) (PubMed).

  • Antigène Voir toutes IL-13 (IL13) Kits ELISA
    IL-13 (IL13) (Interleukin 13 (IL13))
    Autre désignation
    IL13 (IL13 Produits)
    Synonymes
    IL13 Kit ELISA, IL-13 Kit ELISA, P600 Kit ELISA, Il-13 Kit ELISA, L13 Kit ELISA, interleukin 13 Kit ELISA, IL13 Kit ELISA, Il13 Kit ELISA
    Sujet

    Protein Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.

    Background: Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.

    Synonyms: Interleukin-13,IL-13,IL13,NC30,

    Full Gene Name: Interleukin-13

    Cellular Localisation: Secreted.
    ID gène
    3596
    UniProt
    P35225
    Pathways
    Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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