ADAM8 Kit ELISA

N° du produit ABIN2859283

Détail du produit ADAM8 Kit ELISA

Antigène: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Épitope: AA 1-497

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM8

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: NSO, M1-P497

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: M1-P497

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed on neutrophils and monocytes.

Plaque: Pre-coated

Protocole: human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
• Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
• Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
• Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Autre désignation: ADAM8 (ADAM8 Produits)

Synonymes: CD156 Kit ELISA, MS2 Kit ELISA, CD156a Kit ELISA, E430039A18Rik Kit ELISA, adam8 Kit ELISA, reprolysin Kit ELISA, si:zc217g15.7 Kit ELISA, zgc:64059 Kit ELISA, ADAM8 Kit ELISA, RGD1566394 Kit ELISA, ADAM metallopeptidase domain 8 Kit ELISA, a disintegrin and metalloproteinase domain 8 Kit ELISA, a disintegrin and metallopeptidase domain 8 Kit ELISA, ADAM metallopeptidase domain 8a Kit ELISA, ADAM8 Kit ELISA, LOC100304737 Kit ELISA, Adam8 Kit ELISA, adam8a Kit ELISA, adam8 Kit ELISA

Sujet:

Protein Function: Possible involvement in extravasation of leukocytes.

Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

Synonyms: Disintegrin and metalloproteinase domain-containing protein 8,ADAM 8,3.4.24.-,Cell surface antigen MS2,CD156a,ADAM8,MS2,

Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 8

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 101

UniProt: P78325

Pathways: Activation of Innate immune Response, M Phase