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Cathepsin L Kit ELISA

CTSL1 Reactivité: Humain AA 113-333 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859284
  • Antigène Voir toutes Cathepsin L (CTSL1) Kits ELISA
    Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
    Épitope
    AA 113-333
    Reactivité
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin L
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    NSO, E113-V333
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: E113-V333
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    human Cathepsin L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin L has been precoated onto 96-well plates. Standards(NSO, E113-V333) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin L amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 477, Standard deviation: 27.7, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 1541, Standard deviation: 92.46, CV(%): 6
    • Sample 3: n=16, Mean(pg/ml): 2626, Standard deviation: 128.7, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 527, Standard deviation: 32.15, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 1712, Standard deviation: 114.7, CV(%): 6.7
    • Sample 3: n=24, Mean(pg/ml): 3015, Standard deviation: 168.84, CV(%): 5.6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Cathepsin L (CTSL1) Kits ELISA
    Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
    Autre désignation
    CTSL (CTSL1 Produits)
    Synonymes
    CATL Kit ELISA, CTSL1 Kit ELISA, MEP Kit ELISA, 1190035F06Rik Kit ELISA, Ctsl1 Kit ELISA, fs Kit ELISA, nkt Kit ELISA, CATHL Kit ELISA, CatL Kit ELISA, Ctsl Kit ELISA, CTSL Kit ELISA, mep Kit ELISA, catl Kit ELISA, ctsl Kit ELISA, cpl-1 Kit ELISA, MGC80629 Kit ELISA, MGC107932 Kit ELISA, LOC663234 Kit ELISA, 26-29-p Kit ELISA, Cp1 Kit ELISA, si:dkey-121a11.6 Kit ELISA, CTSV Kit ELISA, catL Kit ELISA, cb15 Kit ELISA, ctsl1b Kit ELISA, hgg1 Kit ELISA, wu:fb30g09 Kit ELISA, zgc:92089 Kit ELISA, cathepsin L Kit ELISA, cathepsin V Kit ELISA, cathepsin L L homeolog Kit ELISA, cathepsin L1 Kit ELISA, Cathepsin L1 Kit ELISA, Cathepsin L Kit ELISA, testin gene Kit ELISA, cathepsin 12 Kit ELISA, cathepsin Lb Kit ELISA, cathepsin L.1 Kit ELISA, CTSL Kit ELISA, Ctsl Kit ELISA, CTSV Kit ELISA, ctsl.L Kit ELISA, ctsl Kit ELISA, LOC100008830 Kit ELISA, catl1 Kit ELISA, 26-29-p Kit ELISA, catl Kit ELISA, Tsp_11596 Kit ELISA, Testin Kit ELISA, cts12 Kit ELISA, LOC102156614 Kit ELISA, ctslb Kit ELISA, ctsl.1 Kit ELISA
    Sujet

    Protein Function: Important for the overall degradation of proteins in lysosomes.

    Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

    Synonyms: Cathepsin L1,3.4.22.15,Cathepsin L,Major excreted protein,MEP,Cathepsin L1 heavy chain,Cathepsin L1 light chain,CTSL,CTSL1,

    Full Gene Name: Cathepsin L1

    Cellular Localisation: Lysosome.
    ID gène
    1514
    UniProt
    P07711
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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