CSF3R Kit ELISA

N° du produit ABIN2859293

Détail du produit CSF3R Kit ELISA

Antigène: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Épitope: AA 25-621

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CSF3R/G-CSF R

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: NSO, E25-P621

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E25-P621

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.

Plaque: Pre-coated

Protocole: human CSF3R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF3R has been precoated onto 96-well plates. Standards(NSO, E25-P621) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF3R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF3R amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CSF3R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 12.51, CV(%): 5.3
• Sample 2: n=16, Mean(pg/ml): 819, Standard deviation: 50, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 1237, Standard deviation: 59.4, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 21.19, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 928, Standard deviation: 66.82, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 1528, Standard deviation: 84.04, CV(%): 5.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Autre désignation: CSF3R (CSF3R Produits)

Synonymes: CD114 Kit ELISA, GCSFR Kit ELISA, Cd114 Kit ELISA, Csfgr Kit ELISA, G-CSF-R Kit ELISA, G-CSFR Kit ELISA, CSF3R Kit ELISA, gcsfr Kit ELISA, cd114 Kit ELISA, colony stimulating factor 3 receptor Kit ELISA, colony stimulating factor 3 receptor (granulocyte) Kit ELISA, colony stimulating factor 3 receptor L homeolog Kit ELISA, granulocyte colony-stimulating factor receptor Kit ELISA, CSF3R Kit ELISA, Csf3r Kit ELISA, csf3r Kit ELISA, csf3r.L Kit ELISA, LOC100433338 Kit ELISA

Sujet:

Protein Function: Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. .

Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

Synonyms: Granulocyte colony-stimulating factor receptor,G-CSF receptor,G-CSF-R,CD114,CSF3R,GCSFR,

Full Gene Name: Granulocyte colony-stimulating factor receptor

Cellular Localisation: Isoform 2: Secreted.

ID gène: 1441

UniProt: Q99062