IGF1R Kit ELISA

N° du produit ABIN2859294

Détail du produit IGF1R Kit ELISA

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Épitope: AA 31-932

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGF1R

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: E31-N932

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E31-N932

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney. .

Plaque: Pre-coated

Protocole: human IGF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF1R has been precoated onto 96-well plates. Standards(NSO, E31-N932) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF1R amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGF1R standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.8, Standard deviation: 0.03, CV(%): 3.7
• Sample 2: n=16, Mean(ng/ml): 2.5, Standard deviation: 0.105, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 4.3, Standard deviation: 0.199, CV(%): 4.6,
• Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.066, CV(%): 5.1
• Sample 2: n=24, Mean(ng/ml): 2.8, Standard deviation: 0.151, CV(%): 5.4
• Sample 3: n=24, Mean(ng/ml): 4.7, Standard deviation: 0.296, CV(%): 6.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: IGF1R (IGF1R Produits)

Synonymes: CMT2N Kit ELISA, CD221 Kit ELISA, IGFIR Kit ELISA, IGFR Kit ELISA, JTK13 Kit ELISA, IGFIRC Kit ELISA, IGFR1 Kit ELISA, IGF-1R Kit ELISA, IGF1R Kit ELISA, DKFZp469I0618 Kit ELISA, igf-1r Kit ELISA, xIGF-1R Kit ELISA, xIGFR Kit ELISA, xigf1r Kit ELISA, A330103N21Rik Kit ELISA, D930020L01 Kit ELISA, hyft Kit ELISA, igf-ir Kit ELISA, IGF-IR Kit ELISA, IGF-IRb Kit ELISA, wu:fb03h06 Kit ELISA, IGF-IRa Kit ELISA, sb:eu1090 Kit ELISA, wu:fi20b10 Kit ELISA, alanyl-tRNA synthetase Kit ELISA, insulin like growth factor 1 receptor Kit ELISA, insulin-like growth factor 1 receptor Kit ELISA, insulin-like growth factor 1 Kit ELISA, insulin-like growth factor I receptor Kit ELISA, insulin like growth factor 1 receptor S homeolog Kit ELISA, insulin-like growth factor 1b receptor Kit ELISA, insulin-like growth factor 1a receptor Kit ELISA, AARS Kit ELISA, IGF1R Kit ELISA, Igf1r Kit ELISA, igf1r Kit ELISA, Igf1 Kit ELISA, LOC780503 Kit ELISA, LOC100136427 Kit ELISA, igf1r.S Kit ELISA, igf1rb Kit ELISA, igf1ra Kit ELISA

Sujet:

Protein Function: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K- driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.

Background: IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Synonyms: Insulin-like growth factor 1 receptor,2.7.10.1,Insulin-like growth factor I receptor,IGF-I receptor,CD221,Insulin-like growth factor 1 receptor alpha chain,Insulin-like growth factor 1 receptor beta chain,IGF1R,

Full Gene Name: Insulin-like growth factor 1 receptor

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 3480

UniProt: P08069

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy