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IL17 Receptor B Kit ELISA

IL17RB Reactivité: Humain AA 18-286 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859296
  • Antigène Voir toutes IL17 Receptor B (IL17RB) Kits ELISA
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Épitope
    AA 18-286
    Reactivité
    • 7
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17RB
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: R18-K286
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: R18-K286
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 SEFIR domain.

    Tissue Specificity: Expressed in several endocrine tissues, mostly in fetal and adult liver, kidney, pancreas, testis, colon, brain and small intestine, not detected in peripheral blood leukocytes, lymphoid organs, and most cell lines.

    Plaque
    Pre-coated
    Protocole
    human IL-17RB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RB has been precoated onto 96-well plates. Standards(NSO, R18-K286) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RB amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-17RB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-17RB standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.43, Standard deviation: 0.053, CV(%): 3.7
    • Sample 2: n=16, Mean(ng/ml): 3.04, Standard deviation: 0.137, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 6.12, Standard deviation: 0.324, CV(%): 5.3,
    • Sample 1: n=24, Mean(ng/ml): 1.86, Standard deviation: 0.106, CV(%): 5.7
    • Sample 2: n=24, Mean(ng/ml): 3.43, Standard deviation: 0.233, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 6.51, Standard deviation: 0.469, CV(%): 7.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes IL17 Receptor B (IL17RB) Kits ELISA
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Autre désignation
    IL17RB (IL17RB Produits)
    Synonymes
    IL17RB Kit ELISA, CRL4 Kit ELISA, EVI27 Kit ELISA, IL17BR Kit ELISA, IL17RH1 Kit ELISA, Evi27 Kit ELISA, IL-17ER Kit ELISA, IL-17Rh1 Kit ELISA, Il17br Kit ELISA, interleukin 17 receptor B Kit ELISA, IL17RB Kit ELISA, Il17rb Kit ELISA
    Sujet

    Protein Function: Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. .

    Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.

    Synonyms: Interleukin-17 receptor B,IL-17 receptor B,IL-17RB,Cytokine receptor-like 4,IL-17 receptor homolog 1,IL-17Rh1,IL17Rh1,Interleukin-17B receptor,IL-17B receptor,IL17RB,CRL4, EVI27, IL17BR,UNQ2501/PRO19612,

    Full Gene Name: Interleukin-17 receptor B

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    ID gène
    55540
    UniProt
    Q9NRM6
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