Kallikrein 12 Kit ELISA

N° du produit ABIN2859315

Détail du produit Kallikrein 12 Kit ELISA

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Épitope: AA 18-248

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK12

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: NSO, A18-N248

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A18-N248

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human KLK12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been precoated onto 96-well plates. Standards (NSO, A18-N248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK12 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1409, Standard deviation: 73.26, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 3882, Standard deviation: 213.51, CV(%): 5.5
• Sample 3: n=16, Mean(pg/ml): 6756, Standard deviation: 405.36, CV(%): 6.0,
• Sample 1: n=24, Mean(pg/ml): 1523, Standard deviation: 89.85, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 4129, Standard deviation: 251.86, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 6526, Standard deviation: 443.76, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Autre désignation: KLK12 (KLK12 Produits)

Synonymes: KLK12 Kit ELISA, 2310008B01Rik Kit ELISA, AI324852 Kit ELISA, KLK-L5 Kit ELISA, KLKL5 Kit ELISA, kallikrein-12 Kit ELISA, kallikrein related-peptidase 12 Kit ELISA, kallikrein related peptidase 12 Kit ELISA, LOC611766 Kit ELISA, Klk12 Kit ELISA, KLK12 Kit ELISA

Sujet:

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Synonyms: Kallikrein-12,3.4.21.-,Kallikrein-like protein 5,KLK-L5,KLK12,KLKL5,UNQ669/PRO1303,

Full Gene Name: Kallikrein-12

Cellular Localisation: Secreted.

ID gène: 43849

UniProt: Q9UKR0

Pathways: Système du Complément