SFTPD Kit ELISA

N° du produit ABIN2859319

Détail du produit SFTPD Kit ELISA

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Épitope: AA 21-375

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625-40.000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SP-D

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A21-F375

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A21-F375

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the SFTPD family.

Tissue Specificity: Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .

Plaque: Pre-coated

Protocole: human SP-D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SP-D has been precoated onto 96-well plates. Standards(NSO, A21-F375) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SP-D amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human SP-D standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 6.32, Standard deviation: 0.537, CV(%): 8.5
• Sample 2: n=16, Mean(ng/ml): 14.3, Standard deviation: 0.729, CV(%): 5.1
• Sample 3: n=16, Mean(ng/ml): 25.4, Standard deviation: 1.7, CV(%): 6.7,
• Sample 1: n=24, Mean(ng/ml): 5.76, Standard deviation: 0.513, CV(%): 8.9
• Sample 2: n=24, Mean(ng/ml): 13.5, Standard deviation: 1.24, CV(%): 9.2
• Sample 3: n=24, Mean(ng/ml): 24.7, Standard deviation: 2, CV(%): 8.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SFTPD (SFTPD Produits)

Synonymes: COLEC7 Kit ELISA, PSP-D Kit ELISA, SFTP4 Kit ELISA, SP-D Kit ELISA, BDE Kit ELISA, BDSD Kit ELISA, HOX4I Kit ELISA, SPD Kit ELISA, AI573415 Kit ELISA, Sftp4 Kit ELISA, surfactant protein D Kit ELISA, homeobox D13 Kit ELISA, surfactant associated protein D Kit ELISA, SFTPD Kit ELISA, HOXD13 Kit ELISA, Sftpd Kit ELISA, SP-D Kit ELISA

Sujet:

Protein Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Synonyms: Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,SFTPD,COLEC7, PSPD, SFTP4,

Full Gene Name: Pulmonary surfactant-associated protein D

Cellular Localisation: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

ID gène: 6441

UniProt: P35247